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    Structured Review

    Bio-Rad triton x 100 bio rad
    Triton X 100 Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 16362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/triton+x+100+bio+rad/Tween+20/pmc13088841-97-42-44
    Average 98 stars, based on 16362 article reviews
    triton x 100 bio rad - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Saline:

    Article Title: The impact of mesenchymal stromal cells on the proliferation and functional maturation of liver organoids
    Article Snippet: .. 3000–5000 Organoids from each passage were gently harvested, washed with Dulbecco’s phosphate‐buffered saline (DPBS; Invitrogen), and fixed in ice‐cold 3.7% formalin (Sigma‐Aldrich) for 20 min. After fixation, samples were washed three times with DPBS containing 1% bovine serum albumin (BSA; Sigma‐Aldrich), 0.1% Triton X‐100 (Bio‐Rad), and 0.05% Tween‐20 (Sigma‐Aldrich) to permeabilize cells and reduce nonspecific binding. ..

    Article Title: Self‐Produced Brain‐Like ECM From 3D‐Cultured Dermal Fibroblasts Enhances Neuronal Growth and Survival
    Article Snippet: MNs cultured in 2D on Day 18 of differentiation and within 3D dermis on Day 38 were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 30 min at 4°C. .. Samples were blocked and permeabilized for 1 h at room temperature in a buffer containing phosphate‐buffered saline (PBS), 0.3% Triton X‐100 (Bio‐Rad), and 5% goat serum (Invitrogen). .. For 2D MNs, cells were incubated overnight at 4°C with specific primary antibodies against anti‐neurofilament M (NF‐M; 1:500; MilliporeSigma) and anti‐Islet‐1 (1:500; Abcam).

    Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Spectrin Leica Biosystems Cat#SPEC1-CE Lamin A/C Vector Laboratories Cat#VP-L550; RRID: AB_2336546 Dystrophin (clone 2C6; MANDYS106) Sigma-Aldrich Cat#MABT827 Pax7 DSHB Cat#PAX7; RRID: AB_528428 Laminin b1 Sigma-Aldrich Cat#MAB1921P; RRID: AB_571039 CD31 (clone 390) eBioscience Cat#14-0311-81; RRID: AB_467200 SV2 (synaptic vesicle glycoprotein 2A) DSHB Cat#SV2; RRID: AB_2315387 Anti-mouse IgG2a 488 Invitrogen Cat#A-21131; RRID: AB_141618 Anti-mouse IgG2b 594 Invitrogen Cat#A-21145; RRID: AB_2535781 Anti-mouse IgG1 488 Invitrogen Cat#A-21121; RRID: AB_2535764 Anti-rat IgG 546 Invitrogen Cat#A-11081; RRID: AB_141738 a-Bungarotoxin Conjugate, Alexa Fluor 488 Invitrogen Cat#B13422 Affini Pure Fab Fragment Donkey Anti Mouse IgG (H + L) Jackson Immuno Research Laboratories Cat#715-007-003; RRID: AB_2307338 Chemicals, peptides, and recombinant proteins Promocell Skeletal Muscle Growth Medium Promocell Cat#C-23060 Penicillin/Streptomycin Gibco Cat#15140122 MegaCell Dulbecco0s Modified Eagle0s Medium Sigma-Aldrich Cat#M3942 Fetal bovine serum (FBS) Gibco Cat#10270-098 b-Mercaptoethanol Gibco Cat#31350-010 MEM Non-Essential Amino Acids Solution (NEAA) Gibco Cat#11140-035 Glutamine (GlutaMAXTM Supplement) Thermo Fisher Cat#35050061 Recombinant FGF-2 PeproTech Cat#100-18B Fibrinogen Sigma Cat#F8630-1G Matrigel Fisher Scientific (Corning) Cat#11543550 DMEM/F12 media Invitrogen Cat#11320-033 Thrombin Sigma Cat#T6634 Paraformaldehyde (PFA) Thermo Scientific Chemicals Cat#043368.9M Goat serum Sigma Cat#G9023 Phosphate Buffered Saline (PBS) Abcam Cat#ab285410 Triton X-100 Bio-Rad Cat1610407 Anti-fade fluorescence mounting medium Abcam Cat#ab104135 DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Sigma Cat#D9542 Iso-pentane Fisher Chemical Cat#P/1030/08 CAS: 78-78-4 SYLGARD 184 Silicone elastomer kit SLS (Dow) Cat#63416.5S Pluronic F-127 Sigma-Aldrich Cat#P2443 UltraPure distilled water Invitrogen Cat#10977-035 Vetergesic (Buprenorphine hydrochloride) National Veterinary Services Cat#28745 (Continued on next page) e1 Cell Reports Medicine 6, 102019, March 18, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Metacam National Veterinary Services Cat#45743 Isoflurane (ISO-VET; Chanelle Pharma) National Veterinary Services Cat#719870 Isoflurane (ISOFLO; Abott Laboratories) National Veterinary Services Cat#115095 Deposited data Bulk RNA-seq of DMD-R3381X and CORR-R3381X myogenic cultures (previously published33) Gene Expression Omnibus GSE159273 Bulk RNA-seq of DMD-K2957fs and CORR-K2957fs myogenic cultures (previously published34) Gene Expression Omnibus GSE189053 Experimental models: Cell lines Human: CORR-R3381X MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin33 N/A Human: CORR-K2957 fs MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin34 N/A Human: hCD133+ (Skeletal muscle-derived CD133+ cells) Medical Research Council Center for Neuromuscular Diseases Biobank ID: 8206 Experimental models: Organisms/strains Mouse: mdx nude (mdxnu/nu) Laboratory of Jennifer E. Morgan8,62 N/A Software and algorithms HALO v3.6.4134 software Indica Labs, Inc N/A GraphPad Prism v10 GraphPad software N/A STAR v2,7.0f Dobin et al.35 https://github.com/alexdobin/STAR R environment v4.2.3 R Core Team https://cran.r-project.org edgeR package v3.40.2 Chen et al.77 McCarthy et al.78 Robinson et al.79 https://bioconductor.org/packages/ release/bioc/html/edgeR.html EnhancedVolcano v1.16.0 Blighe et al.80 https://github.com/kevinblighe/ EnhancedVolcano ComplexHeatmap v2.14.0 Gu et al.81 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html gProfiler Kolberg et al.36 https://biit.cs.ut.ee/gprofiler/gost fgsea package v1.24.0 Korotkevich et al.82 https://bioconductor.org/packages/ release/bioc/html/fgsea.html Other Biopsy punch (5mm) Stiefel Cat#D5245 Scalpel (size 11) Swann-Morton Cat#0503 Vicryl Rapide Sutures Ethicon Cat#W9913 Gum tragacanth Sigma Cat#G-112 Cryostat Leica Cat#CM1850 UV Cell DIVE Multiplex Slide Scanner Leica Microsystems N/A Multistainer Leica Cat#ST5020 Coverslipper Leica Cat#CV5030 Cork disks Fisher Scientific Cat#50-316-04 Coverslips VWR International Cat#631-0138 Slides VWR Cat#631-0108 Corning Costar TC-Treated Multiple Well Plates Merck Cat#CLS3527 (Continued on next page) Cell Reports Medicine 6, 102019, March 18, 2025 e2 OPEN ACCESS

    Binding Assay:

    Article Title: The impact of mesenchymal stromal cells on the proliferation and functional maturation of liver organoids
    Article Snippet: .. 3000–5000 Organoids from each passage were gently harvested, washed with Dulbecco’s phosphate‐buffered saline (DPBS; Invitrogen), and fixed in ice‐cold 3.7% formalin (Sigma‐Aldrich) for 20 min. After fixation, samples were washed three times with DPBS containing 1% bovine serum albumin (BSA; Sigma‐Aldrich), 0.1% Triton X‐100 (Bio‐Rad), and 0.05% Tween‐20 (Sigma‐Aldrich) to permeabilize cells and reduce nonspecific binding. ..

    Article Title: The impact of mesenchymal stromal cells on the proliferation and functional maturation of liver organoids.
    Article Snippet: 3000-5000 Organoids from each passage were gently harvested, washed with Dulbecco’s phosphate‐buffered saline (DPBS; Invitrogen), and fixed in ice‐cold 3.7% formalin (Sigma‐Aldrich) for 20 minutes. .. After fixation, samples were washed three times with DPBS containing 1% bovine serum albumin (BSA; Sigma‐Aldrich), 0.1% Triton X‐100 (Bio‐Rad), and 0.05% Tween‐20 (Sigma‐Aldrich) to permeabilize cells and reduce nonspecific binding. ..

    Immunofluorescence:

    Article Title: 1,3,6,7‐Tetrahydroxy‐8‐prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages
    Article Snippet: The luminescence intensities of firefly luciferase and Renilla luciferase were measured with SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA), and the transcriptional activity was determined by the ratio of firefly luciferase to Renilla luciferase. .. NF‐κB immunofluorescence After being incubated on chamber slides overnight, RAW264.7 cells were pretreated with TPX (20 μM) for 1 h and then stimulated with LPS (1 μg · mL −1 ) for 1 h. The cells were fixed immediately in 4% formaldehyde for 20 min, washed with PBS (pH 7.4) and then permeabilized with 0.5% Triton X‐100 (Bio‐Rad) for 20 min at room temperature. ..

    Incubation:

    Article Title: 1,3,6,7‐Tetrahydroxy‐8‐prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages
    Article Snippet: The luminescence intensities of firefly luciferase and Renilla luciferase were measured with SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA), and the transcriptional activity was determined by the ratio of firefly luciferase to Renilla luciferase. .. NF‐κB immunofluorescence After being incubated on chamber slides overnight, RAW264.7 cells were pretreated with TPX (20 μM) for 1 h and then stimulated with LPS (1 μg · mL −1 ) for 1 h. The cells were fixed immediately in 4% formaldehyde for 20 min, washed with PBS (pH 7.4) and then permeabilized with 0.5% Triton X‐100 (Bio‐Rad) for 20 min at room temperature. ..

    Article Title: 1,3,6,7‐Tetrahydroxy‐8‐prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages
    Article Snippet: The luminescence intensities of firefly luciferase and Renilla luciferase were measured with SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA), and the transcriptional activity was determined by the ratio of firefly luciferase to Renilla luciferase. .. After being incubated on chamber slides overnight, RAW264.7 cells were pretreated with TPX (20 μM) for 1 h and then stimulated with LPS (1 μg · mL −1 ) for 1 h. The cells were fixed immediately in 4% formaldehyde for 20 min, washed with PBS (pH 7.4) and then permeabilized with 0.5% Triton X‐100 (Bio‐Rad) for 20 min at room temperature. ..

    other:

    Article Title: The Drosophila adult midgut progenitor cells arise from asymmetric divisions of neuroblast-like cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER B2 546 HCR amplifier Molecular Instruments N/A B2 647 HCR amplifier Molecular Instruments N/A B3 488 HCR amplifier Molecular Instruments N/A B4 488 HCR amplifier Molecular Instruments N/A B4 647 HCR amplifier Molecular Instruments N/A Software and algorithms Fiji Fiji https://imagej.net/Fiji/Downloads Zen Blue v2.3 Carl Zeiss Inc. N/A Zen Black v2.3 Carl Zeiss Inc. N/A Cellranger v4-0.0 and v3.0.2 10x Genomics https://www.10xgenomics.com/ support/software/cell-ranger/latest R The R Foundation https://www.r-project.org/ Seurat v4.1.1 Hao et al.90 https://satijalab.org/seurat/; RRID: SCR_016341 RMagic (v2.0.3) van Dijk et al.91 https://github.com/cran/Rmagic Monocle v3_1.0.0 Trapnell et al.19 http://cole-trapnell-lab.github.io/ monocle-release/

    Gene Expression:


    Cryo-EM Sample Prep:


    Mass Spectrometry:


    Knock-Out:


    Recombinant:


    Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Spectrin Leica Biosystems Cat#SPEC1-CE Lamin A/C Vector Laboratories Cat#VP-L550; RRID: AB_2336546 Dystrophin (clone 2C6; MANDYS106) Sigma-Aldrich Cat#MABT827 Pax7 DSHB Cat#PAX7; RRID: AB_528428 Laminin b1 Sigma-Aldrich Cat#MAB1921P; RRID: AB_571039 CD31 (clone 390) eBioscience Cat#14-0311-81; RRID: AB_467200 SV2 (synaptic vesicle glycoprotein 2A) DSHB Cat#SV2; RRID: AB_2315387 Anti-mouse IgG2a 488 Invitrogen Cat#A-21131; RRID: AB_141618 Anti-mouse IgG2b 594 Invitrogen Cat#A-21145; RRID: AB_2535781 Anti-mouse IgG1 488 Invitrogen Cat#A-21121; RRID: AB_2535764 Anti-rat IgG 546 Invitrogen Cat#A-11081; RRID: AB_141738 a-Bungarotoxin Conjugate, Alexa Fluor 488 Invitrogen Cat#B13422 Affini Pure Fab Fragment Donkey Anti Mouse IgG (H + L) Jackson Immuno Research Laboratories Cat#715-007-003; RRID: AB_2307338 Chemicals, peptides, and recombinant proteins Promocell Skeletal Muscle Growth Medium Promocell Cat#C-23060 Penicillin/Streptomycin Gibco Cat#15140122 MegaCell Dulbecco0s Modified Eagle0s Medium Sigma-Aldrich Cat#M3942 Fetal bovine serum (FBS) Gibco Cat#10270-098 b-Mercaptoethanol Gibco Cat#31350-010 MEM Non-Essential Amino Acids Solution (NEAA) Gibco Cat#11140-035 Glutamine (GlutaMAXTM Supplement) Thermo Fisher Cat#35050061 Recombinant FGF-2 PeproTech Cat#100-18B Fibrinogen Sigma Cat#F8630-1G Matrigel Fisher Scientific (Corning) Cat#11543550 DMEM/F12 media Invitrogen Cat#11320-033 Thrombin Sigma Cat#T6634 Paraformaldehyde (PFA) Thermo Scientific Chemicals Cat#043368.9M Goat serum Sigma Cat#G9023 Phosphate Buffered Saline (PBS) Abcam Cat#ab285410 Triton X-100 Bio-Rad Cat1610407 Anti-fade fluorescence mounting medium Abcam Cat#ab104135 DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Sigma Cat#D9542 Iso-pentane Fisher Chemical Cat#P/1030/08 CAS: 78-78-4 SYLGARD 184 Silicone elastomer kit SLS (Dow) Cat#63416.5S Pluronic F-127 Sigma-Aldrich Cat#P2443 UltraPure distilled water Invitrogen Cat#10977-035 Vetergesic (Buprenorphine hydrochloride) National Veterinary Services Cat#28745 (Continued on next page) e1 Cell Reports Medicine 6, 102019, March 18, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Metacam National Veterinary Services Cat#45743 Isoflurane (ISO-VET; Chanelle Pharma) National Veterinary Services Cat#719870 Isoflurane (ISOFLO; Abott Laboratories) National Veterinary Services Cat#115095 Deposited data Bulk RNA-seq of DMD-R3381X and CORR-R3381X myogenic cultures (previously published33) Gene Expression Omnibus GSE159273 Bulk RNA-seq of DMD-K2957fs and CORR-K2957fs myogenic cultures (previously published34) Gene Expression Omnibus GSE189053 Experimental models: Cell lines Human: CORR-R3381X MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin33 N/A Human: CORR-K2957 fs MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin34 N/A Human: hCD133+ (Skeletal muscle-derived CD133+ cells) Medical Research Council Center for Neuromuscular Diseases Biobank ID: 8206 Experimental models: Organisms/strains Mouse: mdx nude (mdxnu/nu) Laboratory of Jennifer E. Morgan8,62 N/A Software and algorithms HALO v3.6.4134 software Indica Labs, Inc N/A GraphPad Prism v10 GraphPad software N/A STAR v2,7.0f Dobin et al.35 https://github.com/alexdobin/STAR R environment v4.2.3 R Core Team https://cran.r-project.org edgeR package v3.40.2 Chen et al.77 McCarthy et al.78 Robinson et al.79 https://bioconductor.org/packages/ release/bioc/html/edgeR.html EnhancedVolcano v1.16.0 Blighe et al.80 https://github.com/kevinblighe/ EnhancedVolcano ComplexHeatmap v2.14.0 Gu et al.81 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html gProfiler Kolberg et al.36 https://biit.cs.ut.ee/gprofiler/gost fgsea package v1.24.0 Korotkevich et al.82 https://bioconductor.org/packages/ release/bioc/html/fgsea.html Other Biopsy punch (5mm) Stiefel Cat#D5245 Scalpel (size 11) Swann-Morton Cat#0503 Vicryl Rapide Sutures Ethicon Cat#W9913 Gum tragacanth Sigma Cat#G-112 Cryostat Leica Cat#CM1850 UV Cell DIVE Multiplex Slide Scanner Leica Microsystems N/A Multistainer Leica Cat#ST5020 Coverslipper Leica Cat#CV5030 Cork disks Fisher Scientific Cat#50-316-04 Coverslips VWR International Cat#631-0138 Slides VWR Cat#631-0108 Corning Costar TC-Treated Multiple Well Plates Merck Cat#CLS3527 (Continued on next page) Cell Reports Medicine 6, 102019, March 18, 2025 e2 OPEN ACCESS

    Mutagenesis:


    Software:


    Modification:

    Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Spectrin Leica Biosystems Cat#SPEC1-CE Lamin A/C Vector Laboratories Cat#VP-L550; RRID: AB_2336546 Dystrophin (clone 2C6; MANDYS106) Sigma-Aldrich Cat#MABT827 Pax7 DSHB Cat#PAX7; RRID: AB_528428 Laminin b1 Sigma-Aldrich Cat#MAB1921P; RRID: AB_571039 CD31 (clone 390) eBioscience Cat#14-0311-81; RRID: AB_467200 SV2 (synaptic vesicle glycoprotein 2A) DSHB Cat#SV2; RRID: AB_2315387 Anti-mouse IgG2a 488 Invitrogen Cat#A-21131; RRID: AB_141618 Anti-mouse IgG2b 594 Invitrogen Cat#A-21145; RRID: AB_2535781 Anti-mouse IgG1 488 Invitrogen Cat#A-21121; RRID: AB_2535764 Anti-rat IgG 546 Invitrogen Cat#A-11081; RRID: AB_141738 a-Bungarotoxin Conjugate, Alexa Fluor 488 Invitrogen Cat#B13422 Affini Pure Fab Fragment Donkey Anti Mouse IgG (H + L) Jackson Immuno Research Laboratories Cat#715-007-003; RRID: AB_2307338 Chemicals, peptides, and recombinant proteins Promocell Skeletal Muscle Growth Medium Promocell Cat#C-23060 Penicillin/Streptomycin Gibco Cat#15140122 MegaCell Dulbecco0s Modified Eagle0s Medium Sigma-Aldrich Cat#M3942 Fetal bovine serum (FBS) Gibco Cat#10270-098 b-Mercaptoethanol Gibco Cat#31350-010 MEM Non-Essential Amino Acids Solution (NEAA) Gibco Cat#11140-035 Glutamine (GlutaMAXTM Supplement) Thermo Fisher Cat#35050061 Recombinant FGF-2 PeproTech Cat#100-18B Fibrinogen Sigma Cat#F8630-1G Matrigel Fisher Scientific (Corning) Cat#11543550 DMEM/F12 media Invitrogen Cat#11320-033 Thrombin Sigma Cat#T6634 Paraformaldehyde (PFA) Thermo Scientific Chemicals Cat#043368.9M Goat serum Sigma Cat#G9023 Phosphate Buffered Saline (PBS) Abcam Cat#ab285410 Triton X-100 Bio-Rad Cat1610407 Anti-fade fluorescence mounting medium Abcam Cat#ab104135 DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Sigma Cat#D9542 Iso-pentane Fisher Chemical Cat#P/1030/08 CAS: 78-78-4 SYLGARD 184 Silicone elastomer kit SLS (Dow) Cat#63416.5S Pluronic F-127 Sigma-Aldrich Cat#P2443 UltraPure distilled water Invitrogen Cat#10977-035 Vetergesic (Buprenorphine hydrochloride) National Veterinary Services Cat#28745 (Continued on next page) e1 Cell Reports Medicine 6, 102019, March 18, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Metacam National Veterinary Services Cat#45743 Isoflurane (ISO-VET; Chanelle Pharma) National Veterinary Services Cat#719870 Isoflurane (ISOFLO; Abott Laboratories) National Veterinary Services Cat#115095 Deposited data Bulk RNA-seq of DMD-R3381X and CORR-R3381X myogenic cultures (previously published33) Gene Expression Omnibus GSE159273 Bulk RNA-seq of DMD-K2957fs and CORR-K2957fs myogenic cultures (previously published34) Gene Expression Omnibus GSE189053 Experimental models: Cell lines Human: CORR-R3381X MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin33 N/A Human: CORR-K2957 fs MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin34 N/A Human: hCD133+ (Skeletal muscle-derived CD133+ cells) Medical Research Council Center for Neuromuscular Diseases Biobank ID: 8206 Experimental models: Organisms/strains Mouse: mdx nude (mdxnu/nu) Laboratory of Jennifer E. Morgan8,62 N/A Software and algorithms HALO v3.6.4134 software Indica Labs, Inc N/A GraphPad Prism v10 GraphPad software N/A STAR v2,7.0f Dobin et al.35 https://github.com/alexdobin/STAR R environment v4.2.3 R Core Team https://cran.r-project.org edgeR package v3.40.2 Chen et al.77 McCarthy et al.78 Robinson et al.79 https://bioconductor.org/packages/ release/bioc/html/edgeR.html EnhancedVolcano v1.16.0 Blighe et al.80 https://github.com/kevinblighe/ EnhancedVolcano ComplexHeatmap v2.14.0 Gu et al.81 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html gProfiler Kolberg et al.36 https://biit.cs.ut.ee/gprofiler/gost fgsea package v1.24.0 Korotkevich et al.82 https://bioconductor.org/packages/ release/bioc/html/fgsea.html Other Biopsy punch (5mm) Stiefel Cat#D5245 Scalpel (size 11) Swann-Morton Cat#0503 Vicryl Rapide Sutures Ethicon Cat#W9913 Gum tragacanth Sigma Cat#G-112 Cryostat Leica Cat#CM1850 UV Cell DIVE Multiplex Slide Scanner Leica Microsystems N/A Multistainer Leica Cat#ST5020 Coverslipper Leica Cat#CV5030 Cork disks Fisher Scientific Cat#50-316-04 Coverslips VWR International Cat#631-0138 Slides VWR Cat#631-0108 Corning Costar TC-Treated Multiple Well Plates Merck Cat#CLS3527 (Continued on next page) Cell Reports Medicine 6, 102019, March 18, 2025 e2 OPEN ACCESS

    Fluorescence:

    Article Title: Engineered human myogenic cells in hydrogels generate innervated vascularized myofibers within dystrophic mouse muscle on long-term engraftment.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Spectrin Leica Biosystems Cat#SPEC1-CE Lamin A/C Vector Laboratories Cat#VP-L550; RRID: AB_2336546 Dystrophin (clone 2C6; MANDYS106) Sigma-Aldrich Cat#MABT827 Pax7 DSHB Cat#PAX7; RRID: AB_528428 Laminin b1 Sigma-Aldrich Cat#MAB1921P; RRID: AB_571039 CD31 (clone 390) eBioscience Cat#14-0311-81; RRID: AB_467200 SV2 (synaptic vesicle glycoprotein 2A) DSHB Cat#SV2; RRID: AB_2315387 Anti-mouse IgG2a 488 Invitrogen Cat#A-21131; RRID: AB_141618 Anti-mouse IgG2b 594 Invitrogen Cat#A-21145; RRID: AB_2535781 Anti-mouse IgG1 488 Invitrogen Cat#A-21121; RRID: AB_2535764 Anti-rat IgG 546 Invitrogen Cat#A-11081; RRID: AB_141738 a-Bungarotoxin Conjugate, Alexa Fluor 488 Invitrogen Cat#B13422 Affini Pure Fab Fragment Donkey Anti Mouse IgG (H + L) Jackson Immuno Research Laboratories Cat#715-007-003; RRID: AB_2307338 Chemicals, peptides, and recombinant proteins Promocell Skeletal Muscle Growth Medium Promocell Cat#C-23060 Penicillin/Streptomycin Gibco Cat#15140122 MegaCell Dulbecco0s Modified Eagle0s Medium Sigma-Aldrich Cat#M3942 Fetal bovine serum (FBS) Gibco Cat#10270-098 b-Mercaptoethanol Gibco Cat#31350-010 MEM Non-Essential Amino Acids Solution (NEAA) Gibco Cat#11140-035 Glutamine (GlutaMAXTM Supplement) Thermo Fisher Cat#35050061 Recombinant FGF-2 PeproTech Cat#100-18B Fibrinogen Sigma Cat#F8630-1G Matrigel Fisher Scientific (Corning) Cat#11543550 DMEM/F12 media Invitrogen Cat#11320-033 Thrombin Sigma Cat#T6634 Paraformaldehyde (PFA) Thermo Scientific Chemicals Cat#043368.9M Goat serum Sigma Cat#G9023 Phosphate Buffered Saline (PBS) Abcam Cat#ab285410 Triton X-100 Bio-Rad Cat1610407 Anti-fade fluorescence mounting medium Abcam Cat#ab104135 DAPI (40,6-Diamidino-2-Phenylindole, Dihydrochloride) Sigma Cat#D9542 Iso-pentane Fisher Chemical Cat#P/1030/08 CAS: 78-78-4 SYLGARD 184 Silicone elastomer kit SLS (Dow) Cat#63416.5S Pluronic F-127 Sigma-Aldrich Cat#P2443 UltraPure distilled water Invitrogen Cat#10977-035 Vetergesic (Buprenorphine hydrochloride) National Veterinary Services Cat#28745 (Continued on next page) e1 Cell Reports Medicine 6, 102019, March 18, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Metacam National Veterinary Services Cat#45743 Isoflurane (ISO-VET; Chanelle Pharma) National Veterinary Services Cat#719870 Isoflurane (ISOFLO; Abott Laboratories) National Veterinary Services Cat#115095 Deposited data Bulk RNA-seq of DMD-R3381X and CORR-R3381X myogenic cultures (previously published33) Gene Expression Omnibus GSE159273 Bulk RNA-seq of DMD-K2957fs and CORR-K2957fs myogenic cultures (previously published34) Gene Expression Omnibus GSE189053 Experimental models: Cell lines Human: CORR-R3381X MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin33 N/A Human: CORR-K2957 fs MPCs (CRISPR-corrected DMD patient PSC line derived myogenic progenitor cells) Laboratory of Yung-Yao Lin34 N/A Human: hCD133+ (Skeletal muscle-derived CD133+ cells) Medical Research Council Center for Neuromuscular Diseases Biobank ID: 8206 Experimental models: Organisms/strains Mouse: mdx nude (mdxnu/nu) Laboratory of Jennifer E. Morgan8,62 N/A Software and algorithms HALO v3.6.4134 software Indica Labs, Inc N/A GraphPad Prism v10 GraphPad software N/A STAR v2,7.0f Dobin et al.35 https://github.com/alexdobin/STAR R environment v4.2.3 R Core Team https://cran.r-project.org edgeR package v3.40.2 Chen et al.77 McCarthy et al.78 Robinson et al.79 https://bioconductor.org/packages/ release/bioc/html/edgeR.html EnhancedVolcano v1.16.0 Blighe et al.80 https://github.com/kevinblighe/ EnhancedVolcano ComplexHeatmap v2.14.0 Gu et al.81 https://bioconductor.org/packages/ release/bioc/html/ComplexHeatmap.html gProfiler Kolberg et al.36 https://biit.cs.ut.ee/gprofiler/gost fgsea package v1.24.0 Korotkevich et al.82 https://bioconductor.org/packages/ release/bioc/html/fgsea.html Other Biopsy punch (5mm) Stiefel Cat#D5245 Scalpel (size 11) Swann-Morton Cat#0503 Vicryl Rapide Sutures Ethicon Cat#W9913 Gum tragacanth Sigma Cat#G-112 Cryostat Leica Cat#CM1850 UV Cell DIVE Multiplex Slide Scanner Leica Microsystems N/A Multistainer Leica Cat#ST5020 Coverslipper Leica Cat#CV5030 Cork disks Fisher Scientific Cat#50-316-04 Coverslips VWR International Cat#631-0138 Slides VWR Cat#631-0108 Corning Costar TC-Treated Multiple Well Plates Merck Cat#CLS3527 (Continued on next page) Cell Reports Medicine 6, 102019, March 18, 2025 e2 OPEN ACCESS



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